Predicting suitable release sites for assisted colonisations: a case study of eastern barred bandicoots

Assisted colonisations are increasingly being used to recover endangered or functionally extinct species. High quality habitat at release sites is known to improve the success of assisted colonisations, but defining high quality habitat can be challenging when species no longer inhabit their historical range. A partial solution to this problem is to quantify habitat use at release sites, and use results to inform assisted colonisation in the future. In this study, we quantified habitat use by the eastern barred bandicoot Perameles gunnii, functionally extinct on the Australian mainland, immediately after translocation to an island ecosystem. The release site, Churchill Island in Westernport, Victoria, Australia, has a mix of open woodlands and open pasture, providing a range of habitat conditions considered appropriate for nesting and foraging. A total of 16 bandicoots were radio-tracked for 30 d immediately post-release. Early survivorship was high (94%), with males found to have larger home ranges and move greater distances from their first nest than females. Males and females initially used structurally complex habitats for nesting and foraging; as they became more established, males moved further from their release point and both sexes increased their use of open habitats during nightly activity. Female home ranges had limited overlap, suggesting intra-sexual territoriality. Males exhibited larger overlapping home ranges. Our results assist in quantifying habitat use of bandicoots immediately post-release and will be used to inform future assisted colonisations of the species to larger islands, in the presence of feral cats

Camera trapping: a contemporary approach to monitoring invasive rodents in high conservation priority ecosystems

Invasive rodent species have established on 80% of the world\u27s islands causing significant damage to island environments. Insular ecosystems support proportionally more biodiversity than comparative mainland areas, highlighting them as critical for global biodiversity conservation. Few techniques currently exist to adequately detect, with high confidence, species that are trap-adverse such as the black rat, Rattus rattus, in high conservation priority areas where multiple non-target species persist. This study investigates the effectiveness of camera trapping for monitoring invasive rodents in high conservation areas, and the influence of habitat features and density of colonial-nesting seabirds on rodent relative activity levels to provide insights into their potential impacts. A total of 276 camera sites were established and left in situ for 8 days. Identified species were recorded in discrete 15 min intervals, referred to as \u27events\u27. In total, 19 804 events were recorded. From these, 31 species were identified comprising 25 native species and six introduced. Two introduced rodent species were detected: the black rat (90% of sites), and house mouse Mus musculus (56% of sites). Rodent activity of both black rats and house mice were positively associated with the structural density of habitats. Density of seabird burrows was not strongly associated with relative activity levels of rodents, yet rodents were still present in these areas. Camera trapping enabled a large number of rodents to be detected with confidence in site-specific absences and high resolution to quantify relative activity levels. This method enables detection of multiple species simultaneously with low impact (for both target and non-target individuals); an ideal strategy for monitoring trap-adverse invasive rodents in high conservation areas

pH Landscapes in a Novel Five-Species Model of Early Dental Biofilm

Despite continued preventive efforts, dental caries remains the most common disease of man. Organic acids produced by microorganisms in dental plaque play a crucial role for the development of carious lesions. During early stages of the pathogenetic process, repeated pH drops induce changes in microbial composition and favour the establishment of an increasingly acidogenic and aciduric microflora. The complex structure of dental biofilms, allowing for a multitude of different ecological environments in close proximity, remains largely unexplored. In this study, we designed a laboratory biofilm model that mimics the bacterial community present during early acidogenic stages of the caries process. We then performed a time-resolved microscopic analysis of the extracellular pH landscape at the interface between bacterial biofilm and underlying substrate.-grown dental plaque. We employed the pH-sensitive ratiometric probe C-SNARF-4 to perform real-time microscopic analyses of the biofilm pH in response to salivary solutions containing glucose. Anaerobic glycolysis in the model biofilms created a mildly acidic environment. Decrease in pH in different areas of the biofilms varied, and distinct extracellular pH-microenvironments were conserved over several hours.-grown bacterial biofilms

Probabilistic Phase Labeling and Lattice Refinement for Autonomous Material Research

X-ray diffraction (XRD) is an essential technique to determine a material's crystal structure in high-throughput experimentation, and has recently been incorporated in artificially intelligent agents in autonomous scientific discovery processes. However, rapid, automated and reliable analysis method of XRD data matching the incoming data rate remains a major challenge. To address these issues, we present CrystalShift, an efficient algorithm for probabilistic XRD phase labeling that employs symmetry-constrained pseudo-refinement optimization, best-first tree search, and Bayesian model comparison to estimate probabilities for phase combinations without requiring phase space information or training. We demonstrate that CrystalShift provides robust probability estimates, outperforming existing methods on synthetic and experimental datasets, and can be readily integrated into high-throughput experimental workflows. In addition to efficient phase-mapping, CrystalShift offers quantitative insights into materials' structural parameters, which facilitate both expert evaluation and AI-based modeling of the phase space, ultimately accelerating materials identification and discovery.Comment: 13 pages, 6 figure

Optical Identification of Materials Transformations in Oxide Thin Films

Recent advances in high-throughput experimentation for combinatorial studies have accelerated the discovery and analysis of materials across a wide range of compositions and synthesis conditions. However, many of the more powerful characterization methods are limited by speed, cost, availability, and/or resolution. To make efficient use of these methods, there is value in developing approaches for identifying critical compositions and conditions to be used as a-priori knowledge for follow-up characterization with high-precision techniques, such as micron-scale synchrotron based X-ray diffraction (XRD). Here we demonstrate the use of optical microscopy and reflectance spectroscopy to identify likely phase-change boundaries in thin film libraries. These methods are used to delineate possible metastable phase boundaries following lateral-gradient Laser Spike Annealing (lg-LSA) of oxide materials. The set of boundaries are then compared with definitive determinations of structural transformations obtained using high-resolution XRD. We demonstrate that the optical methods detect more than 95% of the structural transformations in a composition-gradient La-Mn-O library and a Ga$_2$O$_3$ sample, both subject to an extensive set of lg-LSA anneals. Our results provide quantitative support for the value of optically-detected transformations as a priori data to guide subsequent structural characterization, ultimately accelerating and enhancing the efficient implementation of $\mu$m-resolution XRD experiments

Morainal Bank Evolution and Impact on Terminus Dynamics During a Tidewater Glacier Stillstand

Sedimentary processes are known to help facilitate tidewater glacier advance, but their role in modulating retreat is uncertain and poorly quantified. In this study we use repeated seafloor bathymetric surveys and satellite‐derived terminus positions from LeConte Glacier, Alaska, to evaluate the evolution of a morainal bank and related changes in terminus dynamics over a 17‐year period. The glacier experienced a rapid retreat between 1994 and 1999, before stabilizing at a constriction in the fjord. Since then, the glacier terminus has remained stabilized while constructing a morainal bank up to 140 m high in water depths of 240–260 m, with rates of sediment delivery of 3.3 Å~ 105 to 3.8 Å~ 105 m3 a−1. Based on repeated interannual surveys between 2016 and 2018, the moraine is a dynamic feature characterized by push ridges, evidence of active gravity flows, and bulldozing by the glacier at rates of up to meters per day. Beginning in 2016, the summertime terminus has become increasingly retracted, revealing a newly emerging basin potentially signaling the onset of renewed retreat. Between 2000 and 2016, the growing moraine reduced the exposed submarine area of the terminus by up to 22%, altered the geometry of the terminus during seasonal advances, and altered the terminus stress balance. These feedbacks for calving, melting, and ice flow likely represent mechanisms whereby moraine growth may delay glacier retreat, in a system where readvance is unlikely.This work was supported by NSF Arctic Natural Sciences Grants OPP—1503910, 1504191, 1504288, and 1504521. National Geographic CP4‐171R‐17 to E. Pettit and J. Nash helped support 2018 cruise logistics.Ye

Experimental evaluation of the wake characteristics of cross flow turbine arrays

One key factor in the exploitation of tidal energy is the study of interactions of turbines when working in tidal turbine farms. The Momentum Reversal and Lift (MRL) turbine is a novel cross flow turbine. The three blades rotate around a common central horizontal axis which is parallel to their own axis and perpendicular to the flow. The novelty of the MRL turbine is that it relies on the combination of both lift and momentum reversal (drag) for energy extraction. Scaled MRL turbine models of 0.164 m in diameter were used to characterise the flow in three different tidal array settings. Detailed maps of axial velocity profiles and velocity deficits downstream of the turbine are presented, enabling the visualisation of characteristic flow patterns. The results show that the MRL generates lower velocity deficits and turbulence intensities in the near wake than those associated with horizontal axis turbines. The downstream wake was not completely symmetrical which was related to the geometry of the device but also due to the flow developed in the flume. Amongst the three array configurations studied, a fence of turbines with the lowest separation provided the highest power output

Biotelemetry marches on: a cost-effective GPS device for monitoring terrestrial wildlife

<div><p>The availability of low-cost wildlife trackers increases the capacity to collect valuable ecological data when research budgets are limited. We converted a commercially available global positioning system (GPS) product into a low-cost tracking device that sends data via the mobile phone network, and assessed its performance under varying conditions. We established a stationary test, deploying devices along a continuum from open urban areas to topographically and structurally complex forested sites. We tested three features of the device: (a) the GPS, by measuring fix success rate, fix precision and horizontal dilution of precision (HDOP), (b) remote download capacity via the mobile phone network and (c) battery drain. Measures of GPS performance demonstrated high fix success rates and precision. HDOP values were influenced by habitat type and topographical position, but generally remained very low, giving an acceptable degree of error for most applications in wildlife research. Devices experienced delayed data transmission at sites with less phone reception, and faster battery drain at sites with denser vegetation. We recorded device malfunctions in 8.2% of the 110 sampling locations, but these were not associated with habitat type or topography. Our device was effective under a wide range of conditions, and the development process we used provides guidance to other researchers aiming to develop cost-effective wildlife trackers. Reducing the financial and labour costs of acquiring high-quality movement data will improve the capacity to increase sample size in animal movement studies.</p></div

Heavy Meson Electromagnetic Mass Differences from QCD

We compute the electromagnetic mass differences of mesons containing a single heavy quark in terms of measurable data using QCD-based arguments in heavy-quark effective theory. We derive an unsubtracted dispersion relation that shows that the mass differences are calculable in terms of the properties of the lowest-lying physical intermediate states. We then consider the problem in the large-$N$ limit, where $N$ is the number of QCD colors. In this limit, we can write a kind of double-dispersion relation for the amplitude required to determine the electromagnetic mass difference. We use this to derive analogs of the Weinberg sum rules for heavy meson matrix elements valid to leading order in $1/N$ and to $O(1/m_Q)$ in the heavy quark expansion. In order to obtain our final result, we assume that the electromagnetic mass differences and sum rules are dominated by the lowest-lying states in analogy with the situation for the $\pi^+$--$\pi^0$ mass difference. Despite the fact that some of the matrix elements appearing in our final result have not yet been accurately measured, we can obtain useful numerical estimates: for example, we obtain (M_{B^+} - M_{B^0})^{EM} \simeq +1.8 \MeV. We argue that our results are accurate to about $30\%$.Comment: 20 pages, plain TeX, 1 uuencoded postscript figur